Biochemistry Cumulative Final Information

The final exam will consist of two parts. Part one will be a standard test that covers the remainder of signal transduction and as much cancer lectures as we get through. The second part will be both multiple choice and short answers that cover the basics that a person finishing from a year of biochemistry should have. The whole test will count as the fourth test. Topics that the cumulative portion of the test will include are:

- pH and buffer questions.

- Amino acids and proteins. This includes knowing the basic characteristics of amino acids, which are acidic, basic, and so on. I will use the three letter designation for the amino acids just as we have all year long. You will be responsible for understanding the basics of a protein, such as the peptide bond, N and C termini and primary, secondary, tertiary and quaternary structure.

- Protein experimentation – SDS and Native PAGE, Size exclusion and ion exchange chromatography and basic western blot information

- Carbohydrates – what is a carbo and how do they differ. How are carbohydrates bonded together and simple anomeric analysis. What is the function of polysaccharides

- Simple Hemoglobin function. Relate the three dimensional structure with the function of the protein.

- Lipids – structure and simple nomenclature.

- Enzymes – the first eight learning objectives

- Kinetics: Straightforward thermodynamics, and kinetic inhibition. Km and Vmax. Define and be able to graph a double reciprocal plot.

- Basics of metabolism, such as why is ATP a high energy molecule and what are the other key metabolic energy molecules.

Use the chapter learning objectives and questions to guide your studies. It is important to me and I assume that is to you as well that you know the basics when you finish a course such as this where these tools are used in all other disciplines.