Biol109_3_28_12
Lab A Group

Info and help for Lab 5: Osmosis - enough said!

McGraw Hill Site with animation on "How Osmosis Works"

This site includes a quiz to test your knowledged of the concept

http://highered.mcgraw-hill.com/sites/0072495855/student_view0/chapter2/animation__how_osmosis_works.html

MCGraw Hill Site with animation on "Hemolysis and Crenation" where you can see the microscopic views of red blood cells cells in isotonic solution, hypertonic solution and hypotonic solution.

http://highered.mcgraw-hill.com/sites/0072495855/student_view0/chapter21/animation__hemolysis_and_crenation.html

 

Most of you need to RUN not walk to the OWL Purdue Online Writing Lab

 

Since some of you are still having trouble using the proper APA format with your in-text citations and reference lists so perhaps this video will be helpful to you.

 

Note: There are lots of videos that will help you learn how to set up MSWord 2010 to make use of the built-in APA fortmatting features.

 

 

 

Info and help for Lab 4: A study of lactose intolerance to further understand the function of enzymes

 

Procedure Modifications for Lab 4: I went through the procedure step by step and added a few additional controls in this handout. I also incorporated a modified table with room to enter the data you collect during the lab.

An Excel doc with a table for the exercise

An Excel doc with a table and fake data to use as a sample to demonstrate graphing.

An Excel doc with a table and fake data that has been plotted to demonstrate a possible way to graph the data.

 

Good animation of Lac operon referred to in handout

Amother site of interest: Drink Milk in Africa? Genes Behind Lactose Tolerance

 

 

Make use of Dr. Dan's Guide to Making Beautiful Lab Reports using Microsoft Word and Excel 2007. This is the site Mazz directed you to earlier in the term and on his webpages.

Remember to include all the points Mazz wants for your lab reports in general (see your course syllabus and the revised general lab report rubric I posted) and to address the specific items mentioned in this new lab 4 handout.

 

Revised General Lab Report Rubric for Lab A

 

Food Allergy vs. food intolerance: What's the difference?

http://www.mayoclinic.com/health/food-allergy/AN01109

Mayo Clinic Lactose Intolerance

http://www.mayoclinic.com/health/lactose-intolerance/DS00530

Mayo Clinic Food Allergy

http://www.mayoclinic.com/health/food-allergy/DS00082

 

Sites I think are helpful and straightforward when it comes to writing scientific reports:

The Writing Center@the University of Wisconsin-Madison gives concise tips and tells you what to avoid Formatting Science Reports

Owl Purdue online Writing Lab's General APA Guidlines

Some changes and some help......

This week you submit your formal lab report based on our first lab. I will grade those and give you some feed back before the third lab.

The second lab requires us to get started right away. While the DNA gel is running, you will begin to answer the Lessons 1-3 questions. We have decided you will not have to write up this lab as a formal report. Instead of submitting a formal report you will:

If we do not get to complete the electrophoresis and take a picture of the gel before the lab ends, I will post the picture of the gel here for you to use in completing the plotting and interpretation of the results sections.

Link to MSWork docx with a picture of the Group A gel.

Click here to open the MSWord document with the gel picture.

The lanes are labeled and I added a white bar across the top of the lanes to indicate where the wells were. Measure down from the white bar to the center of each band in the molecular weight control lane to determine the distance migrated in millimeters (mm) and use thee values to plot your standard curve. Since we let the gel run longer our fragments migrated further and you will need to change the numbers on the axises labeled Distance, mm on each graph. Change the marked units on the graph on page 17 from 0, 5, 10, 15, 20, 25, 30 and 35 to 0, 10, 20, 30, 40, 50, 60, and 70. change the marked units on the graph on page 18 from 10,12,14,16,18, 20, 22, 24, 26, 28 and 30 to 20, 24, 28, 32, 36, 40, 44, 48, 52, 56 and 60.

Visual Aid

Running an Agarose Gel - University of Leicester

Sizing DNA by agarose gel electrophoresis

Fred Hutchinson Research Cancer Center

Cherry Hill High School EAST

Interactive animations

You can "create a DNA Fingerprint and determine which sister did it at this PBS NOVA site:

http://www.pbs.org/wgbh/nova/teachers/body/create-dna-fingerprint.html

 

Here is another interactive animation on DNA Fingerprinting:

http://www2.edc.org/weblabs/DNAfingerprinting/DNAfingerprintingMenu.html

Even more if you are keen

More info on DNA Forensics at Human Genome Project Information